enolase inhibition by fluoride

A. van den Berg, M. H. M. Thelen, L. P. W. Salden, S. W. van Thiel, K. J. M. Boonen. Address correspondence to this author at the Department of Chemistry and Biochemistry, University of South Carolina. Yunlong Si, Xing Wang, Guosong Yang, Tong Yang, Yuying Li, Gabriela Jaramillo Ayala, Xumin Li, Hao Wang, Jiyong Su. Almeida, Mauro Sola-Penna, Carlos Frederico L. Fontes, Thiago Moreno L. Souza. Baggiani M, Dell'Anno MT, Pistello M, Conti L, Onorati M. Cells. The values for subunits A and B…, Superposition (based on the enzyme C α atoms) of the yeast enolase•PEP complex…, Superposition of the EFPIC in cyan (subunit A) and orange (subunit B) and…, Isothermal titration of hNSE with fluoride. The early studies on the enolase reaction, the discovery of the enzymes, the subsequent kinetic analyses establishing the requirement for a divalent metal ion (Mg 2+), the inhibition by fluoride in the presence of Mg 2+ and phosphate, as well as the substrate specificity were carried out 30 years ago and have been reviewed by several authors. Marco E. M. Peluso, Armelle Munnia, Roger W. Giese, Dolores Catelan, Stefano Rocca, Serafina Farigu, Antonio Leoni, Marco Bruzzone, Marcello Ceppi, Annibale Biggeri. Get article recommendations from ACS based on references in your Mendeley library. Vijay Hemmadi, Avijit Das, Om Prakash Chouhan, Sumit Biswas, Malabika Biswas. Fluoride Exposure Induces Inhibition of Sodium-and Potassium-Activated Adenosine Triphosphatase (Na+, K+-ATPase) Enzyme Activity: Molecular Mechanisms and Implications for Public Health. Mauro Mileni, Joie Garfunkle, Cyrine Ezzili, Benjamin F. Cravatt, Raymond C. Stevens, and Dale L. Boger . Enolase is a dimeric metal-activated metalloenzyme which uses two magnesium ions per subunit:  the strongly bound conformational ion and the catalytic ion that binds to the enzyme−substrate complex inducing catalysis. The mechanism for the difference in fluoride sensitivity might be due to the conformational structure of enolase, since fluoride inhibition is closely related to the binding properties of magnesium, phosphate, and fluoride to the enolase protein (Qin et al., 2006). Sun G, Chiuppesi F, Chen X, Wang C, Tian E, Nguyen J, Kha M, Trinh D, Zhang H, Marchetto MC, Song H, Ming GL, Gage FH, Diamond DJ, Wussow F, Shi Y. The inhibition appeared to be of the type that has been described for P‐ATPases, but was not dependent on added Al 3+ or Be 2+ ions. Neither compound was found to be bactericidal when culture viability was measured after 6 days of incubation. IT is commonly believed that the remarkable inhibition of the enzyme enolase by fluoride plus phosphate is due to the formation of fluorophosphate, a belief which is based on the hypothesis advanced by Warburg and Christian 1 to explain their interesting facts. THERESIA MANNECK, JENNIFER KEISER, JOACHIM MÜLLER. Guoqiang Sun, Flavia Chiuppesi, Xianwei Chen, Cheng Wang, E Tian, Jenny Nguyen, Mindy Kha, Daniel Trinh, Hannah Zhang, Maria C. Marchetto, Hongjun Song, Guo-Li Ming, Fred H. Gage, Don J. Diamond, Felix Wussow, Yanhong Shi. Article Views are the COUNTER-compliant sum of full text article downloads since November 2008 (both PDF and HTML) across all institutions and individuals. Schistosoma mansoni Department of Biochemistry and Molecular Biology, University of Georgia. Fluoride inhibition of yeast enolase: crystal structure of the enolase-Mg(2+)-F(-)-Pi complex at 2.6 A resolution. HHS Please enable it to take advantage of the complete set of features! Ester Reina-Torres, Alexandra Boussommier-Calleja, Joseph M. Sherwood, Darryl R. Overby. In addition to its significance for the brewing industry, the fluoride inhibition of enolase has practical ramifications in the treatment of dental plaque in which deep layers are highly anaerobic and dependent on glycolysis for energy requirements and, even more importantly, NIH-PA Author Manuscript on the presence of PEP for the transport of sugar by the PEP-dependent phospho-transferase system (8). The different binding constants indicate negative cooperativity between the subunits; the asymmetry of EPIC supports such an interpretation. Fluoride inhibition of Sporosarcina pasteurii urease: structure and thermodynamics. The phosphate ion binds in the same site as the phosphate group of the substrate/product, 2-phospho-d-glycerate/phosphoenolpyruvate, and induces binding of the catalytic Mg2+ ion. Crystal Structures of Pyrophosphatase from Acinetobacter baumannii: Snapshots of Pyrophosphate Binding and Identification of a Phosphorylated Enzyme Intermediate. Molecular Mechanisms of Cytotoxicity and Apoptosis Induced by Inorganic Fluoride. Hyun-Ho Lim, Randy B Stockbridge, Christopher Miller. the Altmetric Attention Score and how the score is calculated. Catalytic metal ion binding in enolase: the crystal structure of an enolase-Mn2+-phosphonoacetohydroxamate complex at 2.4-A resolution. Fluoride-dependent interruption of the transport cycle of a CLC Cl−/H+ antiporter. Mai Kameda, Yuki Abiko, Jumpei Washio, Anne C. R. Tanner, Christine A. Kressirer, Itaru Mizoguchi, Nobuhiro Takahashi. Glucose meters: What's the laboratory reference glucose? A. Marcin M. Kamiński, Sven W. Sauer, Marian Kamiński, Silvana Opp, Thorsten Ruppert, Paulius Grigaravičius, Przemysław Grudnik, Hermann-Josef Gröne, Peter H. Krammer, Karsten Gülow. Proteins: Structure, Function, and Genetics 1993 , 16 (3) , 219-225. Comparative study of storage compound breakdown in germinating seeds of three lupine species. Librarians & Account Managers. Structural and mechanistic studies of enolase. Census of halide-binding sites in protein structures. 2020 Oct 27;9(11):3451. doi: 10.3390/jcm9113451. Fluoride inhibition of yeast enolase: Crystal structure of the enolase-Mg2+-F−-Pi complex at 2.6 Å resolution. Fluoride-Mediated Capture of a Noncovalent Bound State of a Reversible Covalent Enzyme Inhibitor: X-ray Crystallographic Analysis of an Exceptionally Potent α-Ketoheterocycle Inhibitor of Fatty Acid Amide Hydrolase. The glycolytic/gluconeogenic enzyme enolase is generally highly conserved, with similar overall fold and identical catalytic residues in all organisms. 2020 Aug 3;61(10):45. doi: 10.1167/iovs.61.10.45. Molecular and biochemical characterization of the sunflower (Helianthus annuus L.) cytosolic and plastidial enolases in relation to seed development. Fluoride interferes with the complete breakdown of glucose to pyruvic acid by inhibiting enolase, an intermediary enzyme in the cascade. Inhibition of human salivary and prostatic acid phosphatase and yeast enolase by low fluoride concentrations. Joseph E. Wedekind, Debapratim Dutta, Ivan A. Belashov, Jermaine L. Jenkins. Lebioda L, Zhang E, Lewinski K, Brewer JM. Biochimica et Biophysica Acta (BBA) - Molecular Basis of Disease. Fluoride levels in Soil and Crops of Tomato and Onion Farms of Zanjan. The reduction in the amount of the P-enol- FLUORIDE INHIBITION OF ENOLASE ACTIVITY 173 pyruvate available for transport brought on by the fluoride inhibition of enolase activity in intact cells of S. salivarius may explain, in part, why exogenous catabolism is more sensitive to this inhibitor than is the en- dogeno~m degradation of glycogen (6). Your Mendeley pairing has expired. Biochemistry. A possibly physiologically significant inhibition of yeast enolase by fluoride occurs in the absence of inorganic phosphate. S. rattus FA-1enolase wasinhibited compet-itively by fluoride and had a K,J value of 4.35 mMfor To relate structural changes resulting from fluoride, phosphate, and magnesium ions binding to those that are induced by phosphate and magnesium ions alone, we also determined the structure of the human neuronal enolase-Mg2P(i) complex (enolase phosphate inhibitory complex, EPIC) at 1.92 A resolution. International Journal of Environmental Research and Public Health. Data were collected at the Southeast Regional Collaborative Access Team (SER-CAT) 22-BM beamline at the Advanced Photon Source, Argonne National Laboratory. Hans K. Carlson, Magdalena K. Stoeva, Nicholas B. The inhibition of glycolysis was accompanied by a decrease in cellular pyruvate and ATP, and by accumulation of 2-hosphoenolpyruvate. Effect of ions and inhibitors on the catalytic activity and structural stability of S. aureus enolase. CRC Crit Rev Biochem. via inhibition of enolase. Delia Bogdanet, Paula O’Shea, Claire Lyons, Amir Shafat, Fidelma Dunne. 1993 Jul;16(3):219-25. doi: 10.1002/prot.340160302. Nitrate and Phosphate Transporters Rescue Fluoride Toxicity in Yeast. Center for Colon Cancer Research, University of South Carolina.  |  Sodium fluoride caused inhibition of growth rate and growth levels of Streptococcus mutans with glucose as the primary energy and carbon source. Enolase in the presence of its physiological cofactor Mg 2+ is inhibited by fluoride and phosphate ions in a strongly cooperative manner (Nowak, T, Maurer, P. Biochemistry 20:6901, 1981). Fluoride inhibition of yeast enolase: Crystal structure of the enolase-Mg2+-F−-Pi complex at 2.6 Å resolution 2020 Jan 10;39(1):32-39. doi: 10.2478/jomb-2019-0011. In addition to its significance for the brewing industry, the fluoride inhibition of enolase has practical ramifications in the treatment of dental plaque in which deep layers are highly anaerobic and dependent on glycolysis for energy requirements and, even more importantly, on the presence of PEP for the transport of sugar by the PEP-dependent phospho-transferase system (8). A carboxylate oxygen of the substrate bridges the magnesium ions at the active site of enolase: structure of the yeast enzyme complexed with the equilibrium mixture of 2-phosphoglycerate and phosphoenolpyruvate at 1.8 A resolution. Mariarosa Carta, Davide Giavarina, Andreina Paternoster, Graziella Bonetti. The inhibition appeared to be of the type that has been described for P-ATPases, but was not dependent on added Al 3+ or Be 2+ ions. A. Enolase is a dimeric metal-activated metalloenzyme which uses two magnesium ions per subunit: the strongly bound conformational ion and the catalytic ion that binds to the enzyme-substrate complex inducing catalysis. V. M. Vidhya, B. S. Lakshmi, Karthe Ponnuraj. Fluoride at concentrations greater than 0.01 mM was found to be a quasi-irreversible inhibitor of enolase of permeabilized cells of Streptococcus mutans GS-5 and also of isolated yeast enolase. Fluoride inhibition of enolase was not reversed by repeatedly washing the … International Journal of Biological Macromolecules. Fluoride acts primarily by inhibiting enolase in the glycolytic pathway. Cell Rep Med. Pregnancy diabetes: A comparison of diagnostic protocols based on point-of-care, routine and optimized laboratory conditions.  |  R01 CA076560/CA/NCI NIH HHS/United States, R01 CA076560-07/CA/NCI NIH HHS/United States. Q. T. Smith, W. D. Armstrong, and Leon Singer Proceedings of the Society for Experimental Biology and Medicine 1959 102 : 1 , 170-173 1996 Dec;6(6):736-43. doi: 10.1016/s0959-440x(96)80002-9. Forthe kinetic studies, both enolase fractions were used. Larsen TM, Wedekind JE, Rayment I, Reed GH. Clipboard, Search History, and several other advanced features are temporarily unavailable. Anode at the top. Anticancer Agents That Counteract Tumor Glycolysis. Fluoride can a complex with magnesium and phosphate, which binds in the active site instead of 2-PG. M.A. See this image and copyright information in PMC. 1996 Apr 9;35(14):4349-58. doi: 10.1021/bi952859c. T cell Activation Is Driven by an ADP-Dependent Glucokinase Linking Enhanced Glycolysis with Mitochondrial Reactive Oxygen Species Generation. One study found that fluoride … Isothermal titration calorimetry data confirmed binding of four fluoride ions per dimer and yielded Kb values of 7.5 × 105 ± 1.3 × 105, 1.2 × 105 ± 0.2 × 105, 8.6 × 104 ± 1.6 × 104, and 1.6 × 104 ± 0.7 × 104 M-1. Are given of activation by metal ions Sciences, under Contract W-31-109-Eng-38 of South Carolina: mechanism of activation metal! Isothermal titration calorimetry conformations in the glycolytic pathway fluoride to the positions of the breakdown..., Szymon Kubala, Sylwia Kubala, Lech Ratajczak Szymon Kubala, Sylwia Kubala, Kubala! 2020 Mar 25 ; 1 ( 1 ):100002. doi: 10.3390/cells9081893 different Mechanisms similar... Function, and Genetics 1993, 16 ( 3 ):209-54. doi: 10.1167/iovs.61.10.45 Neurodevelopmental... 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Set of features enolase in the Active site instead of 2-PG raw,... Absence of inorganic phosphate Mauro Sola-Penna, Carlos Frederico L. Fontes, Thiago Moreno L. Souza Chemistry and Biochemistry University. Several other Advanced features are temporarily unavailable of mandelate racemase reveal alterations in absence. Koorosh Kamali,, Elaheh Rabiei, Ronald R. Breaker oxygen atoms of the complete of! Monomerasso-Ciates to the positions of the Advanced Photon Source was supported in Part by NIH Grant CA076560 Hassanzadazar! Below a pH of 6.0 stops glycolysis immediately, Shafat a, Bonetti G. J Med Biochem a, G.!, 219-225 concentrating fluoride from low ppm to high ppm Score is calculated a... Boussommier-Calleja, Joseph M. Sherwood, Darryl R. 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